小鼠骨髓、脾脏的免疫细胞参考值（C57BL/6NCr、BALB/cAnNCr和129/SvHsd，根据品系和性别统计）

下图为临床前研究中常用的三种小鼠品系（C57BL/6NCr、BALB/cAnNCr和129/SvHsd）中关键免疫细胞类型，根据品系和性别统计的均数分布。

a图显示的是 C57BL/6NCr、BALB/cAnNCr 和 129/SvHsd 小鼠骨髓中 CD4+ 和 CD8+ T 细胞、Treg、B 细胞、NK 细胞、iMC（未成熟粒细胞）、pDC、mDC、巨噬细胞和中性粒细胞在不同品系小鼠和不同性别中的比例。

b图是 C57BL/6NCr、BALB/cAnNCr 和 129/SvHsd 脾脏中 CD4+ 和 CD8+ T 细胞、Treg、B 细胞、NK 细胞、iMC（未成熟粒细胞）、pDC、mDC、巨噬细胞和中性粒细胞在不同品系小鼠和不同性别中的比例。

a和b图中，上面三个为雄鼠，下面三个为雌鼠。

各类免疫细胞的设门逻辑如下： 

CD4+ T cells: P1 = live cells, P2 = CD3 APC-Cy7, and CD4 eFluor450 double-positive gate.

CD8+ T cells: P1 = live cells, P2 = CD3 APC-Cy7 and CD8 FITC double-positive gate.

CD4+ Tregs: P1 = live cells, P2 = CD3 APC-Cy7 and CD4 eFluor450 double-positive gate, P3 (intracellular staining) = CD25 PE and FoxP3 APC double-positive gate.

B cells: P1 = live cells, P2 = CD19 PE, and B220 Vio770 double-positive gate.

NK cells: P1 = live cells, P2  = CD3 APC-Cy7 negative, and NKp46 FITC positive gate.

Immature myeloid cells (iMCs): P1 = live cells, P2  = CD11b VioBlue and Gr-1 APC-eFluor780 double-positive gate, P3  = F/80 APC negative and Ly6b FITC negative, F4/80 APC positive and Ly6b FITC negative, F4/80 APC positive and Ly6b FITC positive gate.

Neutrophils: P1 = live cells, P2 = CD11b VioBlue and Gr-1 APC-eFluor780 double-positive gate, P3 = F4/80 APC negative and Ly6b positive gate.

Macrophages: P1 = live cells, P2 = CD11b VioBlue and F4/80 APC double-positive gate, P3 = CD68 PE (intracellular staining) positive histogram gate.

mDC: P1 = live cells, P2 = CD11b VioBlue and CD11c PE double-positive gate.

pDC: P1 = live cells, P2  = CD11b VioBlue negative and CD11c PE positive gate, P3 = B220 Vio770 and Siglec H FITC double-positive gate.

信源：Hensel, J.A., Khattar, V., Ashton, R. et al. Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations. Lab Invest 99, 93–106 (2019). https://doi.org/10.1038/s41374-018-0137-1

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