清华大学免疫学医学院转染BMDMs细胞发表文章到JEM已见刊

一、2020第一季度，清华大学免疫学与医学院；清华大学生命科学学院联合发表标题为：The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens的文章到JEM；

二、用invigentech（英克)INVI DNA RNA Transfection Reagent转染试剂；

三、成功转染BMDMs细胞(小鼠骨髓来源的巨噬细胞)发表文章见刊；

四、文章简介：结肠巨噬细胞转录因子RBP-J协调肠道对细菌的免疫病原体研究.

五、文章部分内容见下：

RNA-mediated interference

siRNA specifically targeting mouse Cebpb and nontargeting control siRNA were purchased from GenePharma Company. siRNA was transfected into BMDMs using INVI DNA RNA Transfection Reagent according to the manufacturer’s in structions (Invigentech). Cells were used 48 h after transfection. 

Figure S2. RBP-J KO mice display normal activation of ILC3 and humoral immune responses during the adaptive immune response phase against C.

rodentium infection. (A) 6–8-wk-old WT and RBP-J KO mice were orally inoculated with 2 × 109 CFUs of C. rodentium, and colons were harvested at the

indicated time points p.i. qPCR analysis of the indicated mRNAs in colon tissues is shown. Data are pooled from two independent experiments; n = 3 in each

group. (B) qPCR of Rbpj in sorted LP CD4+ T cells from uninfected mice. Data are pooled from two independent experiments; n = 3 in each group. (C–H)

Representative FACS plots of colonic LP ILC3 (CD45midCD3−Thy-1+), IL-22–producing ILC3 and Th17 cells (CD3+CD4+RORγt+) at day 7 p.i. (C, E, and G) and

cumulative data (D, F, and H). Data are pooled from two independent experiments; n ≥ 3 in each group. (I and J) Serum anti–C. rodentium immunoglobulin G2b

(IgG2b) and IgG2c titers (I) and fecal secretory IgA (sIgA) titers (J) at day 12 p.i. were measured by ELISA. Data are pooled from two independent experiments;

n = 3 in each group. Data are shown as mean ± SEM; two-tailed Student’s unpaired t test. n.s., not significant; SSC-A, side scatter area.

invigentech（英克）INVI DNA转染试剂信息如下

www.ivigt.com